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Case Study B2 - Resolution
 

TraQ Program of the BC PBCO

Sunday, 20 August 2017

Case Study B2 - Resolution

ABO grouping*

* Reverse group with 4 drops of patient plasma and 1 drop of red cells, incubated at RT.

ABO
    anti-A anti-B A1 cells B cells Interpretation
RT IS 0 4+ 0 0 invalid**
Inc. 15' 0 4+ 1+ 0 invalid**

RT - room temperature  IS- immediate spin

** according to transfusion service SOP

The following enhancement was done:

* Reverse group with 2 drops of patient plasma and 1 drop of red cells, incubated at 4° C for 15 minutes

 

ABO grouping
    anti-A anti--B A1  
cells

cells
Interpretation
RT IS 0 4+ 0 0 invalid
4° C 15' 0 4+ 2+ 0 B

 

Controls - ABO grouping
Cells* RT - IS 4° C - 15'
Screen Cell 1 0 0
Screen Cell 2 0 0
Screen Cell 3 0 0
Autocontrol 0 0

* The autocontrol can detect if a cold autoantibody such as autoanti-I is present (which would react with both A1 and B cells). The group O screen cells is included to detect possible cold-reactive alloantibodies such as anti-N, -P1, and -Lea (whose corresponding antigens may be present on either or both of the  A1 and B cells).

The patient's ABO group was determined to be group B.

Last modified on Wednesday, 19 January 2011 16:35