6.1.5.Review each column under the antigen. If there is a sufficient number of cells, as defined in the following table, to exclude the corresponding antibody, place an "x" above the antigen.
Note: Every laboratory sets its own protocol for antibody exclusion. The ones here are taken from the TraQ Technical Resource Manual and constitute but one example of good practice.
|Antibody Specificity||Minimum Number of Cells Required|
|Anti-C, -E, -c, -e
Anti-S, -s, -M, -N
|1 homozygous cell
(2 preferred, if available)
In the presence of anti-D, 2 heterozygous examples of C and E are considered sufficient for exclusion.
|Anti-K, -k||2 heterozygous or 1 homozygous cell|
|Anti-f Anti-P1, Anti-Lea, -Leb Anti-Xga||Any 1 cell|
|Anti-Cw, -V Anti-Kpa , -Jsa Anti-Lua Anti-Wra||Routine exclusion is not usually required unless an antibody directed to a low frequency antigen is suspected (i.e. the panel cell, carrying the antigen, is positive and there are no other antibodies or explanations for the positive result).|